In the world of analyzing DNA through real-time PCR and multiplex, we can’t underestimate the significance of purified and high-quality DNA isolation systems. Finding the most suitable DNA isolation systems so as to satisfy your needs for downstream application is important to complete experiments successfully. In this guide, we will address how whole plasmid sequencing works, challenges during production, applications, and benefits.
How It Works
Novel Nanopore technology helps to get long sequences of a few kilobases. It is powerful enough to analyze and characterize plasmids, which are circular and small DNA pieces that independently replicates genome. Other benefits of using the technology includes the following:
- High resolution – It provides higher resolution data that allows researchers precisely characterize and identify subtle differences in whole plasmid sequences.
- High accuracy – The technology has a high accuracy. This makes it more suitable for various applications, like quality control, plasmid engineering, and identification of mutating genetics.
- Long-read lengths – It generates continuous and long reads, which are helpful for assembling more complex structures or for sequencing larger plasmids.
- Versatility – The technology is suitable for different types of samples, including degraded and low-quality DNA, making it the most versatile tool for analyzing plasmid.
- High throughput – It allows high-throughput sequencing of several samples in just a single run. This makes it more suitable for large-scale projects and studies.
Challenges Facing Production for Plasmid
Managing reagents that are plasmid-based is challenging. Unlike management of compounds where researchers manage samples through liquid transfers and dilution of synthesized materials, reagents are expanded or regenerated in the sample management facilities. Because sequencing of whole plasmids involve multiple steps, it is also challenging to automate the entire process. At some point, breakdown in quality can render the results of the test useless.
Applications
Researchers use whole plasmid sequencing in clinical microbiology, which is the study of plasmid dynamics in the microbial communications. This helps them understand genetic elements and how resistance in antibiotic genes spread so they can develop better strategies of treatment. The procedure is also applied in environmental monitoring to assess the effects of anthropogenic activities in microbial populations as well as the diversity in genetic to facilitate development of more eco-friendly strategies of managing waste. Lastly, the procedure applies in biotechnology. It serves as diverse goals of biotechnology, including genetic therapy, protein production, and different biotechnological applications, like ensuring safety of GMO and verifying genetic changes.
Why Whole Plasmid Sequencing
Through chemistry of improved library prep, the procedure can easily characterize the whole circular plasmid with just a single read. These long reads historically were not that accurate compared to short reads, requiring more costly and lengthier procedure to get the same data. However, improvement in chemistry makes long reads more accurate and faster methods for sequencing plasmids. Other benefits of whole plasmid sequencing includes:
- Faster sequencing
- Possible to skip primer steps
The bottom line is that whole plasmid sequencing is a procedure, which is more than a tool. For researchers, the procedure is a game-changer, especially in genomics. Its real power lies in its capability to offer comprehensive and accurate information while revolutionizing researchers’ daily work.